Software that supports S/BLOW5

Basecalling

• Guppy via buttery-eel
• Dorado (through our fork)
• Bonito (through our fork)
• slorado
• RODAN
• radian

Methylation calling

• Remora on Guppy with buttery-eel
• Dorado (through our fork)
• f5c (version 0.8 onwards)
• nanopolish (version 0.14.0 onwards)

raw signal mapping/alignment

• sigmap
• OpenDBA
• sigfish and HARU
• DeepSelectNet
• SquiggleNet
• RawHash
• UNCALLED4

raw signal manipulation

• sigtk
• slow5tools
• slow5curl

conversion

• slow5tools
• blue-crab

raw signal simulation

• squigulator

raw signal visualisation

• squigualiser
• nanoCEM