ONT R9.4.1 chemistry - RNA Data
Following are datasets uploaded by others. Some of them have very old basecalls.
In some of them, raw FAST5 signal files are no longer working with latest Guppy.
I have converted them to BLOW5 format and have basecalled using a very recent Guppy through buttery-eel.
GM12878 MinION cDNA data from Nanopore WGS Consortium
- link: https://github.com/nanopore-wgs-consortium/NA12878/blob/master/RNA.md
- associated publication: https://www.nature.com/articles/s41592-019-0617-2
- raw signal data converted to BLOW5 format can be obtained from SRR23513622. filename:
na12878_cDNA_blow5.tar (md5sum: cba2ce651d8c33528e594a9e45ff6515)
- Guppy 6.1.3 high accuracy rebasecalled reads (through buttery-eel wrapper, with dna_r9.4.1_450bps_hac.cfg model) that passed the default qscore filter: SRR23513619.
GM12878 MinION direct-RNA data from Nanopore WGS Consortium
- link: https://github.com/nanopore-wgs-consortium/NA12878/blob/master/RNA.md
- associated publication: https://www.nature.com/articles/s41592-019-0617-2
- raw signal data converted to BLOW5 format can be obtained from from SRR23513624. filename:
na12878_directRNA_blow5.tar (md5sum: 282e305f2b6a72d28980a8d5c803d54e. Also available for direct download from na12878_rna_merged.blow5 (md5sum: 36bc164e9d885838245073f6cd2ecd79), na12878_rna_merged.blow5.idx (md5sum: 82f96208ac2f42574abe0cf5a3954602)
- Guppy 6.1.3 high accuracy rebasecalled reads (through buttery-eel wrapper, with rna_r9.4.1_70bps_hac.cfg model) that passed the default qscore filter: SRR23513623.
DNA datasets from Singapore Nanopore-Expression Project (SG-NEx)