seq

ONT R10.4.1 4kHz chemistry - DNA data

NA24385 (HG002) PromethION data (~30X)

• SRA: https://www.ncbi.nlm.nih.gov/sra/?term=SRS16575602
• associated publication: https://www.nature.com/articles/s41587-021-01147-4
• info: sheared DNA libraries (~17Kb) were prepared using the ONT LSK114 ligation library prep and a R10.4.1 flow cell was used to generate ~30X genome coverage.
• Notes: the complete dataset with 15.3M reads (102.2 Gbases), as well as a subset of 500,000 reads are available

Complete Dataset (15.3M reads):

raw signal data:

Description	SRA accession	Direct download link (md5sum)
BLOW5 format	SRR23215366	PGXX22394_reads.blow5 (3498b595ac7c79a3d2dce47454095610), PGXX22394_reads.blow5.idx (1e11735c10cf63edc4a7114f010cc472)

basecalls:

Basecaller	model	Notes	SRA accession
Guppy 6.3.7	dna_r10.4.1_e8.2_sup@v3.5.1	live basecalling; reads that passed the default qscore filter	SRR23215363
Guppy 6.1.5	dna_r10.4.1_e8.2_hac@v3.5.1	reads that passed the qscore filter for threshold 9	SRR23215364

Subset (500,000 reads):

Description	SRA run Data access	Direct download link (md5sum)
BLOW5 format	SRR23215365	hg2_subsample_slow5.tar(65386e1da1d82b892677ad5614e8d84d)

More dataset links

• NA12878 (HG001) PromethION data (~20X): See the gtgseq repository.
• human methylated and non-methylated (WGA) DNA datasets from zymo dna methylation standards (D5013): see ENA under PRJEB64592
• Zymo GridION: see here